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Studies of the Composition and Function of Urine after Oral Administration of Pteroyl-L-glutamic Acid to Rats.
Oral administration of pteroyl-L-glutamic acid (PTG) to rats resulted in the accumulation of PTG in urine. Urine, at 1 and 3 h, contained PTG at levels of 0.16% and 1.0% w/v, respectively, and recovered at about 10-fold higher levels, 4.9 and 17.3% w/v, after 48 h. It is concluded that PTG is eliminated from the body via a route(s) other than the intestine. The hypothesis that the epithelial cells of the bladder are the likely route of excretion of PTG is tested. Male and female rats were given oral PTG and bromocresol green (BCG) which has a similar molecular weight to PTG, and then the quantity of the two compounds remaining in the urine was measured at intervals for 24 h. It was found that, when male rats were given a single dose of PTG, 24 h after its administration the quantity of PTG recovered from the urine was greater than the amount of BCG recovered. This result was interpreted to indicate that ac619d1d87
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